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Knauer HPLC Columns for UHPLC, HPLC, and FPLC


KNAUER offers roughly 6,500 columns for HPLC, UHPLC, and FPLC separations as well as preparative scale columns for purification work. You can use the column selection guide to help find a column or get more detailed information by following the links below:
 
Click on the list below for information on the columns of interest:
 

Eurospher I: the HPLC Columns for Every Day
Eurospher II: the Logical Choice
Eurospher II Chiral
Eurokat: Valuable for Food Analysis
Eurosil Bioselect: Ideal for the Denaturative Separation of Biomolecules
AppliChrom® Columns for SEC and GPC
Sepapure® FPLC Columns
KNAUER Columns From Other Suppliers

Click here for the column selection guide
 
Click here for general information regarding columns from Knauer
 

Eurospher I: the HPLC Columns for Every Day

Knauer Columns


Eurospher I is a silica-based HPLC packing material for a wide range of analytical, semi-preparative and process scale separations. It is ideally suited for high resolution normal phase and reversed phase chromatography. With more than 20 years on the market, the Eurospher stationary phase has earned a reputation as a stable and reliable partner for daily separation tasks.
 
Eurospher I silica features an outstanding mechanical stability, a high available surface area and a narrow particle size distribution.These properties give Eurospher a long lifetime and high loading capacity.
 
Available in many different modifications and column sizes, Eurospher I columns adapt easily to your application and truly strike a balance between efficiency, price and performance.
 
Eurospher I Modifications
 
The Eurospher I stationary phase is available in 7 different surface modifications for a wide range of applications, including reversed phase and normal phase applications. Every Eurospher I modification offers high chemical stability and loading capacity. Our long experience and knowledge in producing HPLC columns ensures you of the highest reproducibility. Every modification must pass extensive quality control procedures, guaranteeing the best batch to batch reproducibility.
 
Click here for more detailed information
 
Click here to download the data sheet
 

top of pageEurospher II: the Logical Choice

Knauer Columns


After more than 20 years on the market, our Eurospher stationary phase has established a reputation for being a first-class packing material for a wide field of applications.
 
Eurospher II covers an even wider range of application areas in classical HPLC, fast HPLC Plus and ultra-fast UHPLC. Based on an ultra pure spherical silica gel, Eurospher II is a high performance column material for analytical, semipreparative and process-scale applications. Eurospher II features very narrow particle and pore size distributions, as well as outstanding mechanical stability. Eurospher II silica gel is perfectly suited to take on routine analyses as well as the most ambitious chromatography tasks.
 
Eurospher II Modifications
 
With the wide range of different surface modifications available, all application fields in reversed phase and normal phase modes are covered. Every Eurospher II modification offers high chemical stability and loading capacity thanks to mono- and multi-functional silanes. With several different levels of endcapping, Eurospher II offers a wide variety of surface modification types for analytical as well as preparative columns. Our long experience and knowledge in producing HPLC columns ensures the highest reproducibility.
 
Click here for more detailed information
 
Click here to download the data sheet
 

top of pageEurospher II Chiral

Knauer Columns


Based on ultra-high purity silica, the Eurospher II Chiral column materials are characterized by high enantioselectivity and loadability. Available in a wide range of dimensions for analytical, preparative and SMB (simulated moving bed) applications, Eurospher II Chiral columns are the perfect choice for even the most challenging enantioseparation tasks
 
Depending on the analytes, three different chiral selectors are available: Amylose-tris-(3,5-dimethylphenylcarbamate), Cellulose-tris-(3,5-dimethylphenylcarbamate) or p-electron donor- and p-electron acceptor groups. Since chiral separations are not always easy to predict, KNAUER offers a chiral screening service for your samples.
 
Click here for more detailed information
 
Click here to download the data sheet
 

top of pageEurokat: Valuable for Food Analysis

Knauer Columns


Eurokat high performance polymer phases were especially developed for the separation of organic acids, carbohydrates, alcohols and even complex mixtures of these compounds. Eurokat is a sulfonated cross-linked styrenedivinylbenzene copolymer available in several ionic forms (H, Ca, Pb and Ag). This particular cation exchanger is characterized by an outstanding density of functional groups, making it the ideal choice for your ion exclusion, size exclusion and ligand exchange chromatography. Eurokat columns require no organic solvents and thus are environmentally friendly.
 
Eurokat Application Areas
 
Four different ionic species are available. Eurokat H is especially designed for the analysis of organic acids and even complex mixtures of acids, sugars and alcohols as well as sugar alcohols. Eurokat Ca and Eurokat Pb are the phases of choice for analysis of smaller carbohydrates (DP < 4), Eurokat Ag is applicable for the separation of sugar oligomers. Eurokat can also be used for the determination of biochemically important molecules such as sugars, artificial sweeteners or acidic metabolic and degradation products.
 
Choice of Eluents
 
Outstanding separation results are achieved isocratically with fresh double-distilled water for Pb or Ca, or Ag type Eurokat columns, while Eurokat H columns perform best when using inorganic acids. Such aqueous eluents produce no harmful waste as with organic solvents such as acetonitrile which necessitate expensive disposal.
 
Click here for more detailed information.
 
Click here to download the data sheet
 

top of pageEurosil Bioselect: Ideal for the Denaturative Separation of Biomolecules

Knauer Columns


The Eurosil Bioselect stationary phase was specifically developed for the separation of proteins and peptides. Eurosil Bioselect columns are available in 3 modifications, useful for a wide range of separation tasks involving large molecules up to 50 000 Da.
 
Strict manufacturing procedures ensure a very narrow pore size distribution. Interfering anions or heavy metal ions on the silica surface are not present in Eurosil Bioselect columns because only ultra pure silica gel and high quality chemical modifications are employed.
 
Eurosil Bioselect Modifications
 
Eurosil Bioselect columns for HPLC feature a wide-pore (300 Å) stationary phase and were specifically designed to separate large biomolecules, such as proteins and peptides.
 
Click here for more detailed information.
 
Click here to download the data sheet
 

top of pageAppliChrom® Columns for GPC/SEC

Applichrom Column


AppliChrom® Columns
 
AppliChrom® GPC/SEC columns come in a range of packing materials allowing their use in GPC/SEC applications in either aqueous or organic media.
 
Click here for more detailed information.
 

top of pageSepapure® Columns for FPLC

Knauer Columns


Prepacked FPLC columns for protein purification based on Agarose and Dextran for all common applications: Size Exclusion Chromotography (SEC), Affinity Chromotography (AC) and Ion-Exchange Chromotography (IEX).
 
The new Sepapure® FPLC column product line offers new high-quality alternatives for Size-Exclusion (SEC), Affinity (AC) and Ion-Exchange chromatography (IEX). The FPLC column line is completing the KNAUER FPLC portfolio and provides the best LC column addition to your FPLC system.
 
The FPLC columns come as prepacked ready-to-use 1 ml or 5 ml cartridge and can also be purchased as bulk resin. The media for affinity chromatography as well as ion-exchange chromatography is based on agarose, which is well-known in FPLC chromatography. For affinity chromatography the agarose solid phase is functionalized with ligands for purification of recombinantly expressed proteins with His- or GST-tags or antibodies via Protein A or Protein G affinity. The Ion-Exchange columns can be purchased as weak and strong anion or cation exchangers. The basis of our FPLC desalting column is dextran, which reliably separates small molecules and salts from valuable proteins.
 
With these materials, the FPLC column Sepapure is providing the best columns to use with your precious AZURA FPLC system, giving you a complete and reliable KNAUER FPLC package.
 
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top of pageKNAUER Columns From Other Suppliers

Knauer Columns


The columns and packing materials listed below are also available from KNAUER. Check the column selection guide or contact us directly for more information.
 
Lichrospher
 
Prontosil
 
Click here for the column selection guide
 
Click here to download the data sheet for ProntoSIL Hypersorb