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Knauer HPLC Columns for UHPLC, HPLC, and FPLC


KNAUER offers roughly 6,000 columns for HPLC, UHPLC, and FPLC separations as well as preparative scale columns for purificationwork. You can use the interactive column finder to search by brand, modification, pore size etc. or get more detailed information by following the links below:
 
Click on the list below for information on the columns of interest:
 

Eurospher I: the HPLC Columns for Every Day
Eurospher II: the Logical Choice
Eurokat: Valuable for Food Analysis
Eurosil Bioselect: Ideal for the Denaturative Separation of Biomolecules
Columns for Gel Permeation Chromatography (GPC)
ECO Glass Columns
ECO PLUS Glass Columns
KNAUER Columns From Other Suppliers

Click here for the interactive column finder
 
Click here for general information regarding columns from Knauer
 

Eurospher I: the HPLC Columns for Every Day

Knauer Columns


Eurospher I is a silica-based HPLC packing material for a wide range of analytical, semi-preparative and process scale separations. It is ideally suited for high resolution normal phase and reversed phase chromatography. With more than 20 years on the market, the Eurospher stationary phase has earned a reputation as a stable and reliable partner for daily separation tasks.
 
Eurospher I silica features an outstanding mechanical stability, a high available surface area and a narrow particle size distribution.These properties give Eurospher a long lifetime and high loading capacity.
 
Available in many different modifications and column sizes, Eurospher I columns adapt easily to your application and truly strike a balance between efficiency, price and performance.
 
Eurospher I Modifications
 
The Eurospher I stationary phase is available in 7 different surface modifications for a wide range of applications, including reversed phase and normal phase applications. Every Eurospher I modification offers high chemical stability and loading capacity. Our long experience and knowledge in producing HPLC columns ensures you of the highest reproducibility. Every modification must pass extensive quality control procedures, guaranteeing the best batch to batch reproducibility.
 
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Click here to download the data sheet
 

top of pageEurospher II: the Logical Choice

Knauer Columns


After more than 20 years on the market, our Eurospher stationary phase has established a reputation for being a first-class packing material for a wide field of applications.
 
Eurospher II covers an even wider range of application areas in classical HPLC, fast HPLC Plus and ultra-fast UHPLC. Based on an ultra pure spherical silica gel, Eurospher II is a high performance column material for analytical, semipreparative and process-scale applications. Eurospher II features very narrow particle and pore size distributions, as well as outstanding mechanical stability. Eurospher II silica gel is perfectly suited to take on routine analyses as well as the most ambitious chromatography tasks.
 
Eurospher II Modifications
 
With the wide range of different surface modifications available, all application fields in reversed phase and normal phase modes are covered. Every Eurospher II modification offers high chemical stability and loading capacity thanks to mono- and multi-functional silanes. With several different levels of endcapping, Eurospher II offers a wide variety of surface modification types for analytical as well as preparative columns. Our long experience and knowledge in producing HPLC columns ensures the highest reproducibility.
 
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Click here to download the data sheet
 

top of pageEurokat: Valuable for Food Analysis

Knauer Columns


Eurokat high performance polymer phases were especially developed for the separation of organic acids, carbohydrates, alcohols and even complex mixtures of these compounds. Eurokat is a sulfonated cross-linked styrenedivinylbenzene copolymer available in several ionic forms (H, Ca, Pb and Ag). This particular cation exchanger is characterized by an outstanding density of functional groups, making it the ideal choice for your ion exclusion, size exclusion and ligand exchange chromatography. Eurokat columns require no organic solvents and thus are environmentally friendly.
 
Eurokat Application Areas
 
Four different ionic species are available. Eurokat H is especially designed for the analysis of organic acids and even complex mixtures of acids, sugars and alcohols as well as sugar alcohols. Eurokat Ca and Eurokat Pb are the phases of choice for analysis of smaller carbohydrates (DP < 4), Eurokat Ag is applicable for the separation of sugar oligomers. Eurokat can also be used for the determination of biochemically important molecules such as sugars, artificial sweeteners or acidic metabolic and degradation products.
 
Choice of Eluents
 
Outstanding separation results are achieved isocratically with fresh double-distilled water for Pb or Ca, or Ag type Eurokat columns, while Eurokat H columns perform best when using inorganic acids. Such aqueous eluents produce no harmful waste as with organic solvents such as acetonitrile which necessitate expensive disposal.
 
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Click here to download the data sheet
 

top of pageEurosil Bioselect: Ideal for the Denaturative Separation of Biomolecules

Knauer Columns


The Eurosil Bioselect stationary phase was specifically developed for the separation of proteins and peptides. Eurosil Bioselect columns are available in 3 modifications, useful for a wide range of separation tasks involving large molecules up to 50 000 Da.
 
Strict manufacturing procedures ensure a very narrow pore size distribution. Interfering anions or heavy metal ions on the silica surface are not present in Eurosil Bioselect columns because only ultra pure silica gel and high quality chemical modifications are employed.
 
Eurosil Bioselect Modifications
 
Eurosil Bioselect columns for HPLC feature a wide-pore (300 Å) stationary phase and were specifically designed to separate large biomolecules, such as proteins and peptides.
 
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Click here to download the data sheet
 

top of pageColumns for Gel Permeation Chromatography (GPC)


PSS SUPREMA
 
PSS SUPREMA gel is a universally applicable polymer column material for the use of aqueous GPC, also in combination with organic solvents like methanol or acetonitrile. The chemical modification of this gel permits its use with a variety of organic mobile phases. The universal application of PSS SUPREMA gel to wide range of polymer types is obtained using a well-balanced ratio of hydrophilic and hydrophobic segments in the polymer network. PSS SUPREMA columns provide the ability to determine the molecular weights of a wide range of polymers including polyacrylic acid, polymethacrylic acid, polysaccharide, cellulose, polyvinyl alcohol, hydrophilic and hydrophilic/hydrophobic block copolymers, polycations, polyanions and also proteins and other biomolecules.
 
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PSS SDV
 
PSS SDV gel is a perfectly spherical, pressure and pH-stable, macroporous styrenedivinylbenzene copolymer network for organic GPC.
 
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top of pageECO Glass Columns

Knauer Columns


The low priced easy-to-handle ECO glass columns are designed for almost all types of purification applications in the low pressure range (max. 10 to 30 bar, depending on internal diameter), their main area of application is in biochromatography.
 
The columns are available in either aqueous buffer or solvent resistant versions and in 5 different lengths and 6 different internal diameters to suit a wide range of applications and for easy upscaling. Plungers are available in two different lengths in order to easily adapt to different bed lengths.
 
The ECO glass columns can be equipped with an optional water-jacket to make them part of our Benchtop Cooling option and can be used over a wide range of temperatures. Packing and refilling of these glass columns is easy and any resin for protein purifications can be used. The columns are supplied with all necessary accessories.
 
Features of ECO Glass Columns:
 


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top of pageECO PLUS Glass Columns

Knauer Columns


The high performance ECO PLUS quick-lock glass columns are made for all kinds of biochromatography applications. Thanks to their extended pressure range, 25 to 80 bar depending on internal diameter, and their easy adaptability, they can be used for a variety of liquid chromatography purification methods.
 
The columns are available in either aqueous buffer or solvent resistant versions and in 3 different lengths and 6 different internal diameters to suit a wide range of applications and for easy upscaling. Plungers are available in two different lengths in order to easily adapt to different bed lengths.
 
Packing and refilling of these glass columns is easy and any resin for protein purifications can be used. The columns are supplied with all necessary accessories.
 
Features of ECO PLUS Glass Columns:
 


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top of pageKNAUER Columns From Other Suppliers

Knauer Columns


The columns and packing materials listed below are also available from KNAUER. Check the interactive column finder or contact us directly for more information.
 
Silica-based Columns:
 


Click here for the interactive column finder
 
Click here to download the data sheet for ProntoSIL Hypersorb